{"id":9728,"date":"2025-12-26T11:32:35","date_gmt":"2025-12-26T06:02:35","guid":{"rendered":"https:\/\/sugarcane.res.in\/?page_id=9728"},"modified":"2025-12-26T11:41:59","modified_gmt":"2025-12-26T06:11:59","slug":"targeted-improvement-of-stress-tolerance-nutritional-quality-and-yield-of-crops-using-genome-editing-in-sugarcane","status":"publish","type":"page","link":"https:\/\/sugarcane.res.in\/?page_id=9728","title":{"rendered":"Targeted improvement of stress tolerance, nutritional quality and yield of crops using Genome Editing in Sugarcane"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"9728\" class=\"elementor elementor-9728\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"has_eae_slider elementor-section elementor-top-section elementor-element elementor-element-a0b23a8 elementor-section-full_width elementor-section-height-default elementor-section-height-default wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no\" data-eae-slider=\"25451\" data-id=\"a0b23a8\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t\t<div class=\"elementor-background-overlay\"><\/div>\n\t\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"has_eae_slider elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-406a852\" data-eae-slider=\"46222\" data-id=\"406a852\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-38e2d9b elementor-widget elementor-widget-heading\" data-id=\"38e2d9b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Targeted improvement of stress tolerance, nutritional quality and yield of crops using Genome Editing in Sugarcane<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t<div class=\"has_eae_slider elementor-element elementor-element-abbd84a e-con-full e-flex wpr-particle-no wpr-jarallax-no wpr-parallax-no wpr-sticky-section-no e-con e-parent\" data-eae-slider=\"35836\" data-id=\"abbd84a\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6fae0bb elementor-widget elementor-widget-text-editor\" data-id=\"6fae0bb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4><strong>Editing of the <\/strong><strong>MATL <\/strong><strong>gene by <\/strong><strong>CRISPR-Cas <\/strong><strong>for <\/strong><strong>haploid induction <\/strong><strong>in sugarcane<\/strong><\/h4><p>The poly cistronic tRNA gRNA method was optimized to edit the matrilineal (MATL) gene in sugarcane using three CRISPR-based DNA constructs. Guide RNAs with high cutting efficiency were selected and combined into a single editing system. The constructs were first tested and then transferred into sugarcane. \u00a0More than 300 transformed plants were screened using DNA-based tests, and two plants were finally confirmed to carry the desired gene edits.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-44b89d6 elementor-widget elementor-widget-image\" data-id=\"44b89d6\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"735\" height=\"239\" src=\"https:\/\/sugarcane.res.in\/wp-content\/uploads\/2025\/12\/recent_research_achievement_improv_24_12_25_no_1.jpg\" class=\"attachment-large size-large wp-image-9705\" alt=\"\" srcset=\"https:\/\/sugarcane.res.in\/wp-content\/uploads\/2025\/12\/recent_research_achievement_improv_24_12_25_no_1.jpg 735w, https:\/\/sugarcane.res.in\/wp-content\/uploads\/2025\/12\/recent_research_achievement_improv_24_12_25_no_1-300x98.jpg 300w, https:\/\/sugarcane.res.in\/wp-content\/uploads\/2025\/12\/recent_research_achievement_improv_24_12_25_no_1-600x195.jpg 600w\" sizes=\"(max-width: 735px) 100vw, 735px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f106f0c elementor-widget elementor-widget-image\" data-id=\"f106f0c\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"716\" height=\"274\" src=\"https:\/\/sugarcane.res.in\/wp-content\/uploads\/2025\/12\/recent_research_achievement_improv_24_12_25_no_2.jpg\" class=\"attachment-large size-large wp-image-9706\" alt=\"\" srcset=\"https:\/\/sugarcane.res.in\/wp-content\/uploads\/2025\/12\/recent_research_achievement_improv_24_12_25_no_2.jpg 716w, https:\/\/sugarcane.res.in\/wp-content\/uploads\/2025\/12\/recent_research_achievement_improv_24_12_25_no_2-300x115.jpg 300w, https:\/\/sugarcane.res.in\/wp-content\/uploads\/2025\/12\/recent_research_achievement_improv_24_12_25_no_2-600x230.jpg 600w\" sizes=\"(max-width: 716px) 100vw, 716px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-77654cd elementor-widget elementor-widget-text-editor\" data-id=\"77654cd\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h5><strong>Inputs from Dr R. Manimekalai, V.P. Sobhakumari and C. Appunu<\/strong><\/h5>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Targeted improvement of stress tolerance, nutritional quality and yield of crops using Genome Editing in Sugarcane Editing of the MATL gene by CRISPR-Cas for haploid induction in sugarcane The poly cistronic tRNA gRNA method was optimized to edit the matrilineal (MATL) gene in sugarcane using three CRISPR-based DNA constructs. Guide RNAs with high cutting efficiency were selected and combined into a single editing system. The constructs were first tested and then transferred into sugarcane. \u00a0More than 300 transformed plants were screened using DNA-based tests, and two plants were finally confirmed to carry the desired gene edits. Inputs from Dr R. Manimekalai, V.P. Sobhakumari and C. Appunu<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-9728","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sugarcane.res.in\/index.php?rest_route=\/wp\/v2\/pages\/9728","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sugarcane.res.in\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sugarcane.res.in\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sugarcane.res.in\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/sugarcane.res.in\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9728"}],"version-history":[{"count":13,"href":"https:\/\/sugarcane.res.in\/index.php?rest_route=\/wp\/v2\/pages\/9728\/revisions"}],"predecessor-version":[{"id":9747,"href":"https:\/\/sugarcane.res.in\/index.php?rest_route=\/wp\/v2\/pages\/9728\/revisions\/9747"}],"wp:attachment":[{"href":"https:\/\/sugarcane.res.in\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9728"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}